PEMERIKSAAN ANGKA LEMPENG TOTAL (ALT) DAN MOST PROBABLE NUMBER (MPN) BAKTERI Escherichia coli PADA ES BATU BALOK DAN ES BATU KRISTAL

Ice cubes are widely used by people. Ice cubes on the market are not necessarily safe
for
consumption of low quality and there are many bacteria, one of which is Escherichia coli. Escherichia
coli can cause various diseases such as diarrhea, nausea and vomiting. Therefore, it is necessary to check
the quality of ice stone based on microbio
logical terms.
.
Objectives
:
Thus, the need for quality checks in
terms of Microbiology, based on ice cubes. This research was conducted on a test to see the value of the
number Plate and Most Probable Number of bacteria Escherichia coli on ice cubes ice cr
ystal stones and
beams in accordance with SNI 7388:2009
.
Methods:
Ice Cube samples from Ponorogo were taken using
the Purposive sampling method. Qualitative test in the form of ALT and quantitative in the form of MPN
.
Results:
From the results obtained as
a result of the number of Total Plate on a sample block of ice cubes
is 4511.67 colonies/ml sample on ice and rock crystal was 3378.33 colonies/ml. Of the Total Plate
Number results for samples of ice cubes Ice and rock crystal beams meet the terms. Most P
robabel
Number of bacteria Escherichia coli on ice cubes beams is 31 colonies/g and ice cubes are crystal 7
colonies/g. From the results of the Most Probable Number in the sample did not qualify based on ISO
7388:2009 Limit of Microbial Contamination in Fo
od namely ALT 1 x 10
-
4 colonies/g and MPN < 3/g.
Conclusions and
suggestions
:
Most of the ice cubes circulating in Ponorogo have not fulfilled the
requirements and it is expected that the makers pay more attention to the ingredients and the process of
maki
ng ice cubes, and for consumers to be more careful in consuming
.