“Uji Daya Hambat Antibakteri ekstrak etanol daun afrika (Vernonia amygdalina Del) terhadap pertumbuhan bakteri Bacillus cereus dan Pseudomonas aeruginosa dengan metode difusi agar”

Medicinal plants are plants that one or more of its parts contain substances
that can be used for medicinal purposes. Some medicinal plants which have the
ability to treat diseases including African leaves. Ethanol extracts of African leaves
are known to contain flavonoid compounds which are known to function as
antibacterial. Because of its antibacterial nature, researchers have wanted to conduct
research on whether African leaves can inhibit the growth of Bacillus cereus and
Pseudomonas aeruginosa bacteria and what is the amount of inhibition produced
from ethanol and african extracts againts bacterial Bacillus cereus and Pseudomonas
aeruginosa.
In this test, the ethanol extract of African leaves was used. The extract was
diluted to a concentration of 40%, 20% and 10% by using a solvent DMSO (Dimetil
Sulfoksida). Then the inhibitory test was carried out on the bacteria Bacillus cereus
and Pseudomonas aeruginosa. The inhibitory data are then analyzed using One Way
ANOVA.
On the test One Way ANOVA Ethanol extract of African leaves can inhibit
bacterial growth Bacillus cereus and Pseudomonas aeruginosa compared to
negative controls DMSO (Dimteil Sulfoksida) with the result that the inhibition zone
diameter is 0,00 mm. The result showed the inhibitory activity of ethanol extract
African leaves in the normality test is obtained significance (p) > 0,05 ; homogeneity
test > 0,05 and ANOVA test significance (p) < 0,05. With an average yield of
inhibition in Bacillus cereus bacteria at a concentration of 10% by 14.60 mm, a
concentration of 20% by 15.93 mm, and at a concentration of 40% by 17, 90 mm. In
the bacterium Pseudomonas aeruginosa at a concentration of 10% by 11.67 mm, a
concentration of 20% by 13.67 mm, and at a concentration of 40% by 15.93 mm. So
that each concentration has a strong bacterial growth inhibition response.
Keywords: inhibitory power, ethanol extract of African leaves, agar diffusion