Peningkatan Jumlah Cemaran Mikroba pada Maskara Merek “X” dengan Waktu Penggunaan Berdasarkan Angka Lempeng Total (ALT).

Mascara is a cosmetic product that can thicken, flex, blacken eyelashes.
Cosmetic eyelash makeup that is used in the long term can lead to contamination
of microbial contamination, it is influenced by the lack of maintaining cleanliness
during use, storage, poor use, and use of mascara more than once. Therefore the
researchers tested how the increase in the amount of microbial contamination in
mascara with the time of use for fourteen consecutive days. according to BPOM
RI Number HK.03. 1.23.07.11.6662. In 2011 the total plate number requirement is
5 x 102 colonies / ml.
The population in this test is the "x" brand mascara in one of the Ponorogo
supermarkets by taking it randomly. The number of samples used in the study
were ten packaging. Five new product packaging, tested using the Total Plate
Number to find out whether the product contamination meets the requirements or
not. If the ALT test results meet the requirements, then continued the test of
increasing microbial contamination by taking five packs of the same brand
mascara used by five different volunteers. Use by rubbing on the right and left
lashes twice for 14 consecutive days.
From the experimental results, the total plate number in the new product
sample is 3,34 x 102
colonies / ml. Further testing, the test of increasing microbial
contamination obtained results of B1 samples 3,5 x 102 colonies / ml, B2 samples
7,5 x 102 colonies / ml, B3 samples 7,0 x 102
colonies / ml, B4 samples 3,9x102
colonies/ ml, and B5 4,0 x 102 colonies / ml sample. Based on the requirements of
BPOM RI Number HK.03. 1.23.07.11.6662. In 2011 the amount of Microbial
Contamination in new product mascara was declared as eligible. While the test for
increasing microbial contamination in mascara used by five volunteers 60%
fulfilled the requirements and 40% did not meet the requirements. Thus it can be
concluded that the mascara product is still feasible to be reused after fourteen
times of use
Keywords: Microbial Contamination, Mascara, ALT