“Pemeriksaan Angka Lempeng Total (ALT) Dan Most Probable Number (MPN) Bakteri Escherichia coli Pada Es Batu Balok Dan Es Batu Kristal”.

Ice cubes are widely used by the community. Ice cubes made from water that
is frozen. For making ice cubes from raw water and boiled water. Ice cubes that are
on the market are not necessarily safe for consumption that the quality is low and
there are many bacteria are one bacteria i.e. Escherichia coli. The bacterium
Escherichia coli is can cause a variety of diseases such as diarrhea, nausea and
vomiting. Thus, the need for quality checks in terms of Microbiology, based on ice
cubes. This research was conducted on a test to see the value of the number Plate and
Most Probable Number of bacteria Escherichia coli on ice cubes ice crystal stones
and beams in accordance with SNI 7388:2009 about a maximum Microbial
Impurities in Food i.e. ALT 1 x 10-4 colonies/ml and MPN< 2 colonies/ml.
On the research of qualitative testing was performed in the form of a
quantitative form of ALT and MPN. Data obtained after the test Number Plate and
Most Probable Number. Furthermore the number of bacteria is calculated by the
calculation of the 30-300 colony for Number Plate Total (ALT) and for the Most
Probable Number (MPN) views on the tables of MPN.
From the results obtained as a result of the number of Total Plate on a sample
block of ice cubes is 4511.67 colonies/ml sample on ice and rock crystal was 3378.33
colonies/ml. Of the Total Plate Number results for samples of ice cubes Ice and rock
crystal beams meet the terms. Most Probabel Number of bacteria Escherichia coli on
ice cubes beams is 31 colonies/g and ice cubes are crystal 7 colonies/g. From the
results of the Most Probable Number in the sample did not qualify based on ISO
7388:2009 Limit of Microbial Contamination in Food namely ALT 1 x 10-4
colonies/g and MPN < 3/g.
Keywords: Ice Cubes and Ice Crystal, Total Plate Count, Most Probable Number